Kaifeng Hu, Ph.D., ETH (Swiss Federal Institute of Technology, Switzerland), 2004
Professor of biological NMR,
State Key Laboratory of Phytochemistry and Plant Resources in West China
Kunming Institute of Botany, CAS
Address: 132 Lanhei Road, Heilongtan, Kunming, Yunnan, 650204 China PR
Tel(office):+86-871-6523-8781
Email: kaifenghu@mail.kib.ac.cn
Ph.D. course given: “Liquid state NMR” in “Organic Structure Analysis”
Education
Ph. D., Swiss Federal Institute of Technology, Zürich (ETH), Switzerland 2004;
M.S., Shanghai Institute of Materia Medica, Chinese Academy of Sciences 1998;
B.S., Beijing Medical University (Peking University Health Science Center). 1995.
Academic appointmetns and Positions
Principle Investigator, NMR group, Kunming Institute of Botany, CAS 2012.3-
Assistant Scientist, NMRFAM, University of Wisconsin 2009-2012.3
Research Fellow, NIDDK, NIH, USA 2005-2008
Major research interest:
Metabolomics, Structural Biology, NMR and LC-MS techniques
Our research mainly focuses on developing and jointly applying liquid state Nuclear Magnetic Resonance （NMR）and LCMS method in the research of Metabolomics and Structure Biology with the following two main goals：
(1) Identification of bioactive equivalent combinatorial components of natural medicines.
(2) Understanding the molecular mechanism of the interactions between bioactive equivalent combinatorial components of natural medicines and their target proteins.
Coupling NMR with LC-MS-SPE, we develop and apply metabolomic protocol for the quick discovery and identification of bioactive compounds, maybe of trace amount, in the crude extract of nature products from traditional Chinese herbs. In addition, we are also interested in discovering key biomarker related to major disease.
For structure biology, our research mainly focuses on the structure and mechanism of the enzymes involved in the nature product biosynthesis and proteins of biological and medical importance. Our goal is to understand the interaction between the enzyme and its substrates, also the interaction between the target protein and small chemicals or antibody.
Instruments:
Established in the year of 2012, the group of “Therapeutic Molecular Basis and Mechanism of Natural Products” is equipped with the state-of-the-art chemical, biological, spectral and computational facilities, including tissuelyser, lyophilizer, vacuum concentrator, Beckman centrifuge, French press, deep freezer, AKTA pure system, 400M, 500M(1.7mm TCI cryoprobe), 600M (QCI cryoprobe) and 800M (QCI cryoprobe) NMR spectrometers and a 24-core workstation.
Publications since 2012:
1. Li Feng, Huan Luo, Zhijian Xu， Zhuo Yang， Guoxin Du， Yu Zhang， Lijing Yu， Kaifeng Hu, Weiliang Zhu， Qingchun Tong， Kaixian Chen，Fujiang Guo*, Cheng Huang*, Yiming Li*, Bavachinin, as a novel natural pan-PPAR agonist, exhibits unique synergistic effects with synthetic PPAR-γ and PPAR-α agonists on carbohydrate and lipid metabolism in db/db and diet-induced obese mice, (2016) Diabetologia, online.
2. Kaifeng Hu*, Williard J. Werner, Kylie D. Allen, and Susan C Wang*, Investigation of enzymatic C-P bond formation using multiple quantum HCP nuclear magnetic resonance spectroscopy, Magnetic Resonance in Chemistry, 53, 267–272, 2015.
3. Li Xu, Xiaohuo Shi and Kaifeng Hu*, Quantification of multiple compounds containing heterogeneous elements in the mixture by one-dimensional nuclear magnetic resonance spectroscopy of different nuclei using a single universal concentration reference, Magnetic Resonance in Chemistry, 52, 779–782, 2014.
4. Amanda Poplawski, Kaifeng Hu, Woonghee Lee, Senthil Natesan, Danni Peng, Samuel Carlson, Xiaobing Shi, Stefan Balaz, John L. Markley and Karen C. Glass*, Molecular insights into the recognition of N-terminal histone modifications by the BRPF1bromodomain. Journal of Molecular Biology, 426(8), 1661-1676, 2014.
5. Woonghee Lee#, Kaifeng Hu#, Marco Tonelli, Arash Bahrami, Elizabeth Neuhardt, Karen C. Glass and John L. Markley*，Fast automated protein NMR data collection and assignment by ADAPT-NMR on Bruker spectrometers. Journal of Magnetic Resonance, 236, 83-88, 2013.
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